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发明公开
CN104502596A 一种慢性肾病诊断试剂盒 无效 - 驳回

一种慢性肾病诊断试剂盒
摘要：
本发明公开了一种慢性肾病诊断试剂盒，包括FGF23单抗隆抗体、生物素标记试剂Sulfo-NHS-LC-Biontin、pH 7.0 0.1M的PBS缓冲溶液、casein盐溶液、链霉素亲和素和底物TMB，FGF23抗体与生物素标记试剂Sulfo-NHS-LC-Biontin标记条件为重量比为1:5～1:12，链霉素亲和素的稀释倍数是1:3000。本发明试剂盒FGF23的最低检测限值为10pg/ml，比常规单抗提高了10倍的灵敏度，对于慢性肾病的早期诊断具有重要意义。
摘要（英）：

The invention discloses a chronic nephrosis diagnosis kit. The chronic nephrosis diagnosis kit comprises an FGF23 monoclonal antibody, a biotin labeling reagent Sulfo-NHS-LC-Biontin, 0.1M of PBS buffer solution with pH being 7.0, a casein salt solution, streptomycin avidin and a substrate TMB, wherein the weight ratio of the FGF23 antibody to the biotin labeling reagent Sulfo-NHS-LC biontin is 1:5 to 1:12, and the dilution ratio of the streptomycin avidin is 1 to 3000. The lowest detection limit of the kit FGF23 is 10pg/ml, the sensitivity is improved by 10 times compared with the conventional monoclonal antibody, and the important significance on the early diagnosis of the chronic nephrosis can be realized.

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