The invention relates to an anti-fatigue healthcare cake and a preparation method thereof. The cake is prepared from the following raw materials in parts by weight: 80 to 120kg of flour, 30 to 50kg of oat powder, 20 to 50kg of highland flour, 0.2 to 3kg of cordyceps sinensis or cordyceps sinensis mycelium, 0.5 to 2kg of silverweed cinquefoil root, 0.5 to 3kg of mhvaca, 0.5 to 3kg of lycium ruthenicum murr, 0.5 to 3kg of tricholoma matsutake, 0.5 to 3kg of yak meat, 0.2 to 2kg of rhodiola rosea, 0.2 to 2kg of conic gymnadenia tuber, 0.3 to 3kg of okra, 0.4 to 4kg of poria cocos, 3 to 10kg of dried cherry tomato, 0.2 to 2kg of allium chinensis, 30 to 75kg of grease, 40 to 80kg of sweetening agent, 15 to 25kg of egg, 3 to 8kg of milk powder, 0.8 to 1.5kg of baking soda, and 0.8 to 1.5kg of ammonium bicarbonate; the raw materials above are preprocessed, mixed and uniformly agitated to form a dough for later use, then the dough is rolled into noodle-shaped strips through a roller, and the noodle-shaped strips are cut into the initial cakes to be baked to obtain the cake. The cake is reasonable in nutrition arrangement and has the anti-fatigue healthcare function, can be also used as a snack food which is convenient to carry, and is suitable for industrial production.