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CN104560725B 基于流式细胞技术高通量筛选产乙醇真菌的试剂盒及其应用 失效 - 权利终止

基于流式细胞技术高通量筛选产乙醇真菌的试剂盒及其应用
摘要：
本发明公开了一种基于流式细胞技术高通量筛选产乙醇真菌的试剂盒及其应用，该试剂盒包括盒体与试剂，还包括细胞培养板、离心管和铜筛，所述的试剂为：试剂A：柠檬酸：6.15g；柠檬酸钠：17.544g；1000mL；试剂B：碘化丙啶（PI）：0.3mg；2,7‑二氢二氯荧光黄双乙酸钠：0.3mg；试剂C：葡萄糖：2g，50mL；试剂D：葡萄糖：12g，50mL；试剂E：酵母粉：0.5g；胰蛋白胨：0.5g；磷酸氢二铵：0.05g；硫酸镁：0.025g，pH 5.5，50mL；试剂F：酵母粉：0.05g；胰蛋白胨：0.05g；磷酸氢二铵：0.05g；硫酸镁：0.025g，pH 5.5，50mL。
摘要（英）：

The invention discloses a flow-cytometry-based kit for high-flux screening of ethanol-producing fungi and application thereof. The kit comprises a box body, reagents, a cell culture plate, a centrifuge tube and a copper screen. The reagents are as follows: 1000 mL of reagent A composed of 6.15g of citric acid and 17.544g of sodium citrate, reagent B composed of 0.3mg of propidium iodide (PI) and 0.3mg of DCFH-DA, 50 mL of reagent C composed of 2g of glucose, 50 mL of reagent D composed of 10g of glucose, 50 mL of reagent E (pH value is 5.5) composed of 0.5g of yeast powder, 0.5g of tryptone, 0.05g of ammonium monoacid phosphate and 0.025g of magnesium sulfate, and 50 mL of reagent F (pH value is 5.5) composed of 0.05g of yeast powder, 0.05g of tryptone, 0.05g of ammonium monoacid phosphate and 0.025g of magnesium sulfate.

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