The invention provides a yeast-enriched identification medium for inhibiting mycetes and a preparation method of the yeast-enriched identification medium. The identification medium comprises the following ingredients by weight percent: 1+/-0.2% of yeast powder, 2+/-0.2% of peptone, 2+/-0.2% of glucose, 2+/-0.2% of agar, 4 +/-0.2% of a stock solution A, 0.1 +/-0.2% of a stock solution B, 2.2 +/-0.2% of sodium propionate and the balance of water, and the natural pH is 6.5 +/-0.2. The preparation method comprises the following steps: step A: preparing a basal medium, the stock solution A and the stock solution B according to the formula; step B: adding yeast powder, peptone, glucose, agar and water respectively according to the formula, uniformly mixing, adding the stock solution A and the stock solution B, then adding sodium propionate, so as to enable the natural pH of the medium to reach to 6.5 +/-0.2, sterilizing for 20 minutes at 121+/-0.2 DEG C, and after sterilization adding chloramphenicol and a stock solution C. By adopting the scheme, the mycete bacteria contamination in nature can be effectively inhibited, and the effects of yeast-enriched cultivation and preliminary identification are achieved.