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发明公开
CN104561281A 一种褐潮藻种检测方法及试剂盒 有权

一种褐潮藻种检测方法及试剂盒
摘要：
本发明涉及一种褐潮藻种检测方法，包括以下步骤：1)设计并合成特异性引物AanF和AanR和探针Aan probe，AanF的序列为AAAGCTCGTAGTTGGATTCCTGG，AanR的序列为GTGTTCAACGCAGGCTTACG，探针Aan probe的序列为CTTGCGATGGTCTATCCT；2)选择标准品藻株，并构建重组质粒标准品；3)构建标准曲线；4)引物AanF、引物AanR、探针Aan probe特异性验证；5)样品检测与结果分析。本发明所述的检测方法可以用于褐潮藻种的定性、定量检测，具有灵敏度高、重现性好、连续可测、操作简便、易于控制、分析时间短等优点。
摘要（英）：

The invention relates to a brown tide algae species detection method which comprises the following steps: 1) designing and synthesizing specific primers AanF and AanR and an Aan probe, wherein the sequence of the AanF is AAAGCTCGTAGTTGGATTCCTGG; the sequence of the AanR is GTGTTCAACGCACGCTTACG; the sequence of the Aan probe is CTTGCGATGGTCTATCCT; 2) selecting a standard algal strain and constructing a recombinant plasmid standard; 3) constructing a standard curve; 4) verifying the specificities of the primers AanF and AanR and the Aan probe; 5) performing sample detection and result analysis. The detection method provided by the invention can be used for qualitative and quantitative detection of a brown tide algae species and has the advantages of high sensitivity, good reproducibility, continuous measurement, good convenience and high simplicity in operation, high easiness in control, short analysis time and the like.

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