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发明公开
CN104583387A 粟酒裂殖酵母突变体的转化体、以及克隆载体 有权

粟酒裂殖酵母突变体的转化体、以及克隆载体
摘要：
本发明提供一种不需要繁杂的分离工序就可生产、回收β-葡糖苷酶的粟酒裂殖酵母突变体的转化体，以及对裂殖酵母属酵母的转化有用的载体。本发明的粟酒裂殖酵母突变体的转化体，是Gsf活性增大且丙酮酸转移酶Pvg1的酶活性下降或失活的粟酒裂殖酵母(Schizosaccharomyces pombe)突变体的转化体，其特征在于，在染色体中或者作为染色体外基因，具有来源于丝状真菌的编码β-葡糖苷酶的结构基因序列以及用于表达该结构基因的启动子序列及终止子序列。进一步，本发明涉及以具备hsp9基因所具有的启动子或ihc1基因所具有的启动子为特征的、对裂殖酵母属酵母的转化有用的克隆载体、表达载体、转化体等。
摘要（英）：

Provided are: a transformant of a Schizosaccharomyces Pombe (S. Pombe) mutant with which beta-glucosidase can be produced and collected without requiring a complicated isolation step; and a vector useful for transforming Schizosaccharomyces-group yeast. The transformant of an S. Pombe mutant exhibits increased Gsf activity, reduced or no pyruvyl transferase (Pvg 1) enzyme activity, and is characterized by having, in a chromosome or as an extrachromosomal gene, a promoter sequence and a terminator sequence for expressing a structural gene sequence for coding beta-glucosidase derived from a filimentous fungus, and said structural genes. Furthermore, the present invention relates to a cloning vector, expression vector, transformant, etc., useful for transforming Schizosaccharomyces-group yeast, characterized by having a promoter having the hsp9 gene or a promoter having the ihc1 gene

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