The invention provides an energy storage battery response capability working condition evaluation method. The energy storage battery response capability working condition evaluation method comprises the following steps of (1) selecting actual operation working conditions of energy storage batteries and confirming an evaluation category of the energy storage battery working condition response capability; (2) selecting to-be-evaluated energy storage batteries in a sampling mode from different factories; (3) reconstructing the actual working conditions of the energy storage batteries and formulating experiment testing working conditions; (4) executing experiment testing working conditions on the to-be-evaluated energy storage batteries and recording the current experiment testing data; (5) judging that whether the experiment testing working conditions are to be executed on N evaluation categories or not, returning back to (3) if yes and comparing energy storage battery performance indexes under the same experiment testing working condition of the batteries of different factories and finishing the working condition evaluation. According to the energy storage battery response capability working condition evaluation method, the working condition applicability evaluation can be comprehensively and accurately achieved in a targeted mode based on the actual working data of an energy storage system starting from important performance categories to which the actual applications paid attention.