The invention relates to a low-temperature aqueous-phase catalyst for lignin phenol derivative hydrodeoxygenation and a preparation method thereof. The catalyst comprises a zeolite molecular sieve HBeta serving as a carrier and a load Ru, wherein the molar ratio of silicon to aluminum is 10-50, and the loading amount of Ru is 0.1 to 2%. The preparation method comprises the following steps: mixing measured aqueous solution of RuCl3 with a zeolite suspension, evaporating water and drying, and then reducing the obtained solid with hydrogen/argon mixed gas. A cyclic hydrocarbon (the conversion rate is greater than 98% and the selectivity is greater than 95%) is prepared at high selectivity under the relatively mild (100-150 DEG C and 1-4MPa) condition, so that the defects that the reaction conditions are harsh and the energy efficiency is low in the refining process of biomass pyrolysis oil can be overcome. The bifunctional catalyst provided by the invention has the advantages of simplicity and easiness in operation in the preparation process, stable physical and chemical properties, and direct application. At low temperatures, the catalyst shows excellent hydrodeoxygenation activity and extremely high selectivity of saturated hydrocarbons, has a simple and environment-friendly reaction process, and can be widely used in the refining of bio-gasoline.