The invention belongs to the field of food processing, and particularly relates to health fish oil jelly and a preparation method thereof. The health fish oil jelly comprises the following raw materials in parts by weight: 2-5 parts of fish oil, 0.01-0.1 part of organic acid, 3-10 parts of xylitol, 0.5-2 parts of honey, 0.01-0.03 part of potassium sorbate, 0.01-0.12 part of an emulsifier, 0.0008-0.0011 part of a stabilizer, 3-10 parts of 100% concentrated fruit juice, 0.05-0.2 part of food-grade glycerinum and 2-8 parts of edible gum, wherein the ratio is mass ratio. According to the health fish oil jelly, the defects that the fish oil is easily oxidized and is not easily stored are overcome; and the fish oil can be well dispersed in a glue system, so as to be used for preparing the jelly, so that the fishy smell of the fish oil is concealed; the acceptability of consumers is improved; and the health fish oil jelly is nutrient and delicious, and is easily digested and absorbed by a human body.