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发明授权
CN104650207B 一种能够防治猪蓝耳病的生物活性分子-抗菌肽Cecropin D 有权

一种能够防治猪蓝耳病的生物活性分子-抗菌肽Cecropin D
摘要：
本发明公开了一种新型的生物活性分子‑抗菌肽Cecropin D在制备抗PRRSV病毒和/或防治猪蓝耳病药物中的应用。首先合成抗菌肽Cecropin D的基因序列引物并表达出抗菌肽Cecropin D，然后通过抗大肠杆菌DH5α实验验证其抗菌活性。进一步，在Marc‑145细胞水平上，通过qRT‑PCR、Western‑Blot、TCID50检测及IFA等方法研究其对HP‑PRRSV的抗病毒作用，进一步在病毒吸附、进入细胞和进入细胞后2h、4h四个过程阐述其抗病毒机制。结果显示，本发明所述方法制得的抗菌肽Cecropin D有很好抗HP‑PRRSV病毒效果，有望成为新型的防治猪蓝耳病的生物活性分子。
摘要（英）：

The invention discloses an application of a novel bioactive molecule-antibacterial peptide Cecropin D in preparation of medicines for resisting PRRSV (porcine reproductive and respiratory syndrome virus) and/or preventing and treating a porcine blue-ear disease. The application comprises the following steps: firstly synthesizing gene sequence primers of an antibacterial peptide Cecropin D and expressing the Cecropin D; and verifying the antibacterial activity through an anti-escherichia coli DH5alpha experiment; furthermore, researching the antiviral effect of the antibacterial peptide Cecropin D on HP-PRRSV through qRT-PCR, Western-Blot, TCID50 detection, IFA and other methods at the Marc-145 cell level; and further explaining the antiviral mechanism of the antibacterial peptide in four processes of virus adsorption, cell entry, two hours after cell entry and four hours after cell entry. The result shows that the antibacterial peptide Cecropin D prepared by the method has a good anti-HP-PRRSV virus effect, and is expected to become a novel bioactive molecule for preventing and treating the porcine blue-ear disease.

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