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CN104673936A 猪传染性胃肠炎病毒N基因RT-qPCR检测方法及其引物和TaqMan探针 无效 - 驳回 转让

猪传染性胃肠炎病毒N基因RT-qPCR检测方法及其引物和TaqMan探针
摘要：
本发明提供一种猪传染性胃肠炎病毒N基因的RT-qPCR检测方法及其引物和TaqMan探针。具体地，本发明提供一组猪传染性胃肠炎病毒N基因RT-qPCR检测方法，采用如序列表SEQ ID No.1所示上游引物，如序列表SEQ ID No.2所示的下游引物以及如序列表SEQ ID No.3所示的TaqMan探针序列。本发明提供了基于上述引物和TaqMan探针的猪传染性胃肠炎病毒N基因RT-qPCR检测方法，该检测方法具有良好的检测敏感性，1×105copies/μL-1×101copies/μL时，Ct值与荧光值呈典型的扩增曲线。同时，该检测方法的重复性好、特异性强，操作方便。
摘要（英）：

The invention provides an RT-qPCR detection method, primers and TaqMan probe for detecting a swine transmissible gastroenteritis virus N gene. Particularly, the invention provides a set of RT-qPCR detection method for detecting the swine transmissible gastroenteritis virus gene and a forward primer shown in SEQ ID No.1 in a sequence table, a reverse primer shown in SEQ ID No.2 in the sequence table, and a TaqMan probe sequence shown in SEQ ID No.3 in the sequence table. The invention provides an RT-qPCR detection method for detecting the swine transmissible gastroenteritis virus N gene on the basis of the primers and the TaqMan probe. The detection method has excellent detection sensitivity, and in the case of 1*10<5>copies/microliter to 1*10<1>copies/microliter, Ct values and fluorescence values show typical amplification curves. At the same time, the detection method has good repeatability, strong specificity and convenience in operation.

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