The invention discloses a method for screening dandelion salt-tolerance mutants. The method comprises the steps that full seeds are selected and cultured; aseptic seedlings are acquired and cut into slices; the slices are subjected to callus induction; embryonic calluses are obtained, sequentially inoculated to a liquid differential medium containing EMS (Ethylmethane Sulfonate) and a solid differential medium containing NaCl, and screened; salt-tolerance test-tube seedlings are obtained; acclimatization and transplant are performed to make the salt-tolerance test-tube seedlings survive; then the salt-tolerance test-tube seedlings are transplanted a greenhouse; and salt-tolerance dandelion plants are obtained with the combination of coastal original saline alkali soil potting saline-alkaline tolerance identification method. The method for screening the dandelion salt-tolerance mutants is simple to operate, good in effect, high in efficiency, and very wide in application prospect, and accelerates a dandelion salt-tolerance breeding course.