The invention discloses a manufacturing process of an automatic train identification system host. The manufacturing process comprises the following steps: single-board production, single-board no-power temperature cycle test at -35 DEG C to 70 DEG C, single-board programming, single-board test, whole assembly, integration test for the first time, high-temperature aging test of the powered-on whole machine at 60 DEG C to 70 DEG C, integration test for the second time, high/low-temperature sampling inspection and quality inspection, as well as packaging and storage. According to the manufacturing process, early failure products are screened out through the high-temperature aging test of the powered-on whole machine and the aging test at a high temperature, so that the products can proceed into the index stable period; the process maximally enables defective products to be exposed in the processes, is high in screening test efficiency, and eliminates early failures of the products before discharging the products from the factory.