The invention discloses a gravure plate, and a manufacturing method and an application of the gravure plate. The gravure plate comprises a substrate and an elastic net wall, wherein the contact angle, to the selected ink, of the surface of the substrate is smaller than a first set threshold value, and the elastic net wall is formed on the surface of the substrate, and is provided with a set grid structure; and the contact angle, to the selected ink, of the surface of the net wall is larger than a second set threshold value, and the second set threshold value is larger than or equal to the first set threshold value. The manufacturing method comprises the steps that polymer is applied to the surface of the substrate, and is cured to form the net wall. Net wall materials re directly printed on the substrate to form the gravure plate, the machining period of the gravure plate can be obviously shortened, and the manufacturing cost is greatly reduced. The edge of the gravure plate is smoother than that of a traditional ink cell type gravure plate, hardness is obviously reduced, needed printing pressure is low, and therefore the substrate can be prevented from being damaged. The net wall materials can be printed on the rigid material, in the use process, ink can be greatly saved, a scraper is not needed, and the longer service life is achieved.