The invention discloses a method for chiral resolution of itraconazole. The method utilizes the supercutical fluid chiral chromatography, conducts elution through a chiral chromatographic column by a mobile phase consists of carbon dioxide and a strong polar organic solvent, and conducts two times of separation and purification so as to obtain four itraconazole optical isomers. According to the method, the process operation is simple, the raw material is easy to obtain, the organic reagent used in the preparation process can be recycled, pollution is hardly generated, the product is high in optical purity, and is suitable for being used as a comparison product for new drug research and development, and very important significance is provided for medical application of further development of the itraconazole.