The invention discloses a kit and device for detecting aneuploidy of chromosomes. The device comprises the following modules: a detection module which is used for carrying out high-flux sequencing on a sample to be detected so as to obtain sequenced data; a first judgment module which is used for calculating the chromosomes in the form of cut windows so as to obtain a Z value of each chromosome and preliminarily judging whether each chromosome has aneuploidy or not according to the Z value of each chromosome; a first calculation module which is used for calculating to obtain a first concentration value of fetus DNA (Deoxyribonucleic Acid) in the sample to be detected; a second calculation module which is used for calculating according to an X chromosome or a methylation method so as to obtain a second concentration value of the fetus DNA in the sample to be detected; a second judgment module which is used for judging whether the first concentration value of the fetus DNA in the sample to be detected and the second concentration value of the fetus DNA in the sample to be detected are fit with a curve (y=x) or not; a first determination module which is used for determining that the copy number of each chromosome has aneuploidy. The detecting device has the advantage that the accuracy of detecting the aneuploidy of the chromosomes is higher.