The invention relates to a carbazochrome sodium sulfonate powder injection for injection and a preparation method thereof. A freeze drying powder injection of carbazochrome sodium sulfonate is basically free of freeze-drying excipients, wherein the content of carbazochrome sodium sulfonate is above 90%. When injection water for the freeze-dried powder injection is dissolved in a carbazochrome sodium sulfonate 10 mg/ml concentration solution, the pH value of the solution ranges from 5.0 to 6.0. The carbazochrome sodium sulfonate powder injection for injection can be applied to bleeding of the urinary system, the upper gastrointestinal tract, the respiratory tract and obstetrical and gynecological diseases, has a remarkable curative effect on bleeding of the urinary system, can be applied to traumatic bleeding and operation bleeding, and has an excellent physicochemical property.