The invention discloses a production method of high protein feed rich in tryptophan. The production method includes: subjecting corynebacterium glutamicum, candida tropicalis, lactobacillus plantarum and bacillus natto to liquid fermentation, mixing fermented liquor rich in metabolites and living bacteria, mixing mixture with soybean meal and the like proportionally, performing solid fermentation, and drying and packaging a fermentation product to obtain the high protein feed higher than 50% in crude protein content and higher than 0.9% in tryptophan content. The production method has the advantages that the process is simple, the fermented liquor is directly mixed with solid material for fermentation, the metabolite, tryptophan, of microorganisms is made use, solid fermentation time is greatly shortened, chances of sundry bacteria contaminating in the solid fermentation process of raw materials are slimmed, energy consumption is low, no waste is discharged in the fermentation process, the feed produced has lowered content of anti-nutritional factors in plant meals, the content of the tryptophan is higher than 0.9%, less exogenous tryptophan is added or the tryptophan is replaced, and cost of the feed is reduced.