The invention provides a manual fuel pump pressing mechanism of a diesel oil filter. The manual fuel pump pressing mechanism comprises a manual fuel pump arranged at the head of the diesel oil filter, a rotation driving device, a rotating shaft and a mounting base, wherein a bottom plate of the mounting base is fixedly connected with the head of the diesel oil filter; the bottom plate is provided with a manual fuel pump holding hole; a first side plate of the mounting base is provided with a through hole; the rotating shaft is in rotating fit with the through hole; the rotating shaft is provided with an eccentric part propping against a pressing surface of the manual fuel pump; one end of the rotating shaft is connected with the rotation driving device. The manual fuel pump pressing mechanism of the diesel oil filter provided by the invention drives the rotating shaft to rotate through the rotation driving device, and makes the eccentric part rotate, then the pressing operation to the manual fuel pump can be realized conveniently without manual pressing to the manual fuel pump, thus the labor intensity of operating personnel is greatly reduced, the pressing time of the manual fuel pump is shortened and the production efficiency can be effectively improved.