The invention provides an automatic calibration system and method for electronic-controlled engine fuel oil parameters. The method comprises the steps that according to three calibration modes of dots, lines and faces, oil injection parameters and the variation range of the oil injection parameters are selected to conduct the experiment design; according to position definition variables of the oil injection parameters on a screen and the input sequence of the variables, a computer operating system API is called to generate the automatic calibration system of a fuel oil injection system and control the calibration process of the engine fuel oil system. The oil injection parameters of the fuel oil system are controlled through the automatic calibration system of the fuel oil injection system, then the whole calibration process of the engine fuel oil system is controlled, and finally the automation of the electric engine calibration process is achieved. Accordingly, an engine can operate within different oil injection parameter combinations under a certain working condition when the oil injection parameters are within a certain range, the power performance, the economical efficiency and the emission performance of the engine under different oil injection parameter combinations can be measured, and the labor intensity of calibration engineers and the experiment error rate are reduced.