The invention relates to a method for carrying out gene edition on GT1-7 cells. The method comprises the following steps: laying the GT1-7 cells in a 24-porous plate; growing the cells in an adherence way, transfecting 42229 plasmid containing Cas9 genes and puromycin resistant genes to enter a GT1-7 cell system; expressing the puromycin genes by virtue of the cell, and screening the 42229 transfection GT1-7 cell by utilizing the puromycin; then removing a culture medium containing the puromycin, replacing the culture medium with a normal high-sugar culture medium, carrying out the amplification culture, digesting and transferring a culture plate into a culture bottle to continuously carry out amplification culture when the GT1-7 cells fully grow on the culture plate, thereby obtaining the Cas9 stable-transfection GT1-7 cell. The GT1-7 cell target gene is precisely edited only by transfecting the specified sgRNA plasmid in real application, the affection of large Cas9 plasmid on the tranfection efficiency can be avoided, and the application prospect is good.