The invention discloses a tumor targeted polypeptide photosensitizer bonding object and belongs to the field of biological medicine. The structure of the tumor targeted polypeptide photosensitizer bonding object is A-B-C-D, wherein A represents a photodynamics treatment photosensitizer, B represents cell-penetrating peptide segments containing positive charges, C represents matrix metalloproteinase specific recognition polypeptide segments, and D represents polypeptide segments containing negative charges. By means of the tumor targeted polypeptide photosensitizer bonding object, the stability of the photodynamics treatment photosensitizer under the physiological condition can be remarkably improved, the toxic and side effect of the photodynamics treatment photosensitizer can be greatly reduced, the characteristics of the photodynamics treatment photosensitizer in crossing cell membranes can be remarkably improved, tumor-targeted characteristics of the photosensitizer are improved, the circulation of the photosensitizer in blood can be achieved, thereby therapeutic medicine can be effectively gathered in a tumor area, tumors can be effectively inhibited, and the tumor targeted polypeptide photosensitizer bonding object has great significance and a wide application prospect in diagnosis imaging and the targeted therapy of the tumors.