The invention discloses a full-automatic nucleic acid molecule hybridization instrument. The instrument is characterized by comprising a shell, a circuit control module, a hybridization platform, a three-dimensional movement platform, a multichannel sample adding piston device, Tip heads and a touchable screen monitor. The full-automatic nucleic acid molecule hybridization instrument has the advantages that the hybridization process is full-automatic, and manual operation is not needed, so that labor force is saved, experiment time is shortened, and artificial factor errors in the experiment process are reduced; by the three-dimensional movement platform, the full-automatic nucleic acid molecule hybridization instrument can move quickly, movement precision is high, and accuracy is also high; multi-person experiments can be carried out at the same time, cross infection can be avoided, and experiment time is shortened; the Tip heads are divided into big Tip heads and small Tip heads as needed, the sucking quantity of each big Tip head is large, after sucking nucleic acid molecules, each big Tip head can be used for continuously implementing accurate sampling five times , so that an experiment process is simplified, and the experiment time is shortened.