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发明公开
CN104975083A 一种快速鉴定烟粉虱MEAM1、MED两隐种的引物及其鉴定方法 有权

一种快速鉴定烟粉虱MEAM1、MED两隐种的引物及其鉴定方法
摘要：
本发明公开了快速鉴定烟粉虱MEAM1、MED两隐种的引物及鉴定方法。主要针对烟粉虱MEAM1、MED两隐种线粒体COI基因序列分别设计一对特异引物：MEAM1隐种为：5’-CACTATAATTATTGCTGTTCCC-3’；5’-ACCTAAGATTAGTGGAAATC-3’；MED隐种为5’-TTAATTAGCAGCGAGGCTGG-3’，5’-AGGAAC GGCAATAATCATAGTAGC-3’；同时针对样品DNA提取进行改进，提供了一套大量单只烟粉虱样品DNA的快速提取方法，能短时间稳定获取适用于两隐种鉴别的大量单只样品DNA。构建了一个分子鉴别烟粉虱MEAM1、MED隐种的双引物PCR扩增体系。本发明可应用于田间外来入侵烟粉虱大量MEAM1、MED隐种间的快速鉴定、田间分布、风险评估，为烟粉虱防治策略的制定提供科学依据。
摘要（英）：

The present invention discloses primers and identification method for rapid identification of two bemisia tabaci cryptic species of MEAM1 and MED. A pair of primers are designed respectively mainly aiming at mitochondrial COI gene sequences of two bemisia tabaci cryptic species of MEAM1 and MED: primers for the MEAM1 cryptic species are: 5'-CACTATAATTATTGCTGTTCCC-3', and 5'-ACCTAAGATTAGTGGAAATC-3'; and primers for the MED cryptic species are 5'TTAATTAGCAGCGAGGCTGG-3', and 5'-AGGAAC GGCAATAATCATAGTAGC-3'. The sample DNA extraction were improved, and a method for rapid extraction of a large number of single bemisia tabaci sample DNA is provided, so as to stably obtain a large number of single bemisia tabaci sample DNA applied to identification of both cryptic species in shortened time. The invention constructs a double primer PCR amplification system for molecular identification of bemisia tabaci cryptic species of MEAM1 and MED. The present invention can be applied in rapid identification, field distribution and risk assessment of a large amount of alien invasive bemisia tabaci cryptic species of MEAM1 and MED in the field, and provides a scientific basis to develop strategies for the prevention and treatment of bemisia tabaci.

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