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发明授权
CN105039556B 转基因大豆MON87769的LAMP检测引物组、试剂盒及检测方法 失效 - 权利终止

转基因大豆MON87769的LAMP检测引物组、试剂盒及检测方法
摘要：
本发明公开了一种转基因大豆MON87769的LAMP检测引物组、试剂盒及检测方法。引物组由6条特异性引物组成，其核苷酸序列如SEQ ID NO：1~6所示。检测试剂盒包括检测引物溶液、具有链置换活性的DNA聚合酶、10×反应缓冲液、dNTPs溶液、显色剂。检测方法是采用特异性引物及具有链置换活性的DNA聚合酶，在63~65℃对样品DNA模板进行扩增，并采用加入显色剂观察颜色变化的方法，判断样品是否含有转基因大豆MON87769成分。本发明不需要特殊仪器，具有快速高效、操作简便、特异性强等特点，适合现场检测。
摘要（英）：

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group, an LAMP detection kit and an LAMP detection method for transgenic soybeans MON87769. The primer group consists of six specific primers, and the nucleotide sequence of the primer group is as shown in SEQ ID NO:1-6; the detection kit comprises detection primer solution, DNA (deoxyribonucleic acid) polymerase with strand displacement activity, 10 times reaction buffer, dNTPs (deoxyribonucleoside triphosphate solution) and a color developing agent; according to the detection method, amplification is performed on a sample DNA template at 63 to 65 DEG C by adopting the specific primers and the DNA polymerase with the strand displacement activity, and by adopting a method that the color developing agent is added to observe changes in color, whether the sample contains the components of the transgenic soybeans MON87769 or not is judged. The detection method disclosed by the invention does not need special instruments, has the characteristics of being fast and efficient, simple in operation, high in specificity and the like, and is suitable for on-site test.

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