The invention discloses a molecular breeding method fast obtaining a large number of transgenosis sedum lineare new species. According to the molecular breeding method, blades, stipes and stems of aseptic seedlings are used as transformation accepters, a plant expression vector of target genes is constructed according to the requirement of the breeding purpose, the target genes are guided into a sedum lineare genome through an agrobacterium tumefaciens mediating method, and a regenerated plant is formed through shoot organogenesis way differentiation; a corresponding antibiotic screening transformant of screening marker genes is added during transformation shoot induction, transformation shoot elongation and rooting, and it is verified that an obtained resistance plant is a transformation plant through Southern hybridization and GUS dyeing verification. According to the method, a large number of target gene transformation sedum lineare new species can be obtained by nine weeks, and the urgent demands for breeding sedum lineare fine strains and innovating and researching genetic resources at present can be met. The molecular breeding method has obvious ecological, economic and social benefit significance in sustainable development of sedum lineare.