The invention belongs to the field of microbial fermentation and provides a medium for fermentation cultivation, especially solid fermentation cultivation, of endophytic fungi, and a cultivation method of the endophytic fungi. The solid fermentation medium comprises a substrate and additional nutrient compositions as well as water, wherein based on mass percentage, the substrate comprises 60% of bran, 10% of soybean cake powder, 10% of rice bran and 10% of corn broken grains; the content of water is 50% of total mass of the substrate; based on 1kg of total mass of the substrate, the additional nutrient compositions comprise 12.96g of sucrose, 4.00g of ammonium nitrate, 12.70g of peptone, 1.50g of monopotassium phosphate, 10.00g of calcium sulfate and 0.48g of magnesium sulfate; and the weight of distilled water is 500g. According to the invention, solid fermentation of tobacco endophytic fungi is carried out and initiated for the first time, an artificial neural network of YCEF005 type tobacco endophytic fungi is obtained for the first time, and the fermented product of the tobacco endophytic fungi is fast and effectively obtained.