The invention discloses an SERS-based newcastle disease virus detection kit and a detection method thereof, and belongs to the field of spectroscopy and molecular diagnosis. The SERS-based newcastle disease virus detection kit comprises a raman probe solution and a nitrocellulose film; the raman probe solution is a solution obtained by coupling nano gold, 3,3'-dithio di(6-nitrobenzoic acid) di-succinic acid imido ester complex and a newcastle disease antibody. The detection method comprises the following steps: dropping a newcastle disease virus on the nitrocellulose film, incubating the nitrocellulose film and the raman probe coupled with the antibody after closing and washing to form an immune complex with a 'solid phase antigen-labeled antibody' half sandwich structure on the nitrocellulose film, and finally performing raman spectrum detection on the immune complex so as to identify an enhanced raman molecular signal of a material to be identified with high specificity. The detection kit and the detection method which are disclosed by the invention are high in specificity, and the detection limit of the newcastle disease virus is 10<3>PFU/mL, and the sensitivity is high.