The invention relates to a preparation method of a vaccarin ointment, and belongs to the field of pharmaceutical preparations. The vaccarin ointment has remarkable effect on recovery of open wounds. The preparation method is characterized by including porphyrizing vaccarin, and screening the vaccarin by a 60-mesh sieve for standby application; heating, by mass, 15% of stearin, 20% of liquid paraffin, 2% of stearic acid and 6% of octadecanol (dosages of the reagents are mass fractions in the ointment) to melt the reagents into oil phase; heating 25% of pure glycerinum and 30% of distilled water to the temperature of 90 DEG C, adding 2% of lauryl sodium sulfate and 0.1% of ethylparaben, and dissolving the mixture into water phase; slowly pouring the water phase into the oil phase, and stirring while adding until condensation to obtain an O/W emulsion; adding 1% of the sieved vaccarin into the emulsion, and stirring to obtain the vaccarin ointment. The vaccarin ointment can be applied to production of drugs or cosmetics.