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CN105112395A 猕猴桃AdPDC1基因与应用 无效 - 驳回

猕猴桃AdPDC1基因与应用
摘要：
本发明公开了猕猴桃AdPDC1基因与应用。该猕猴桃AdPDC1是具有序列表中的SEQIDNO.2所述氨基酸序列的蛋白质，其编码基因为AdPDC1基因SEQIDNO.1的DNA序列。猕猴桃AdPDC1基因受涝害、盐害诱导表达。过量表达AdPDC1的转基因拟南芥与野生型组相比，耐涝性能力显著增强。说明猕猴桃AdPDC1在植物提抗涝害的过程中起着重要的作用。另外，ABA处理条件下，转猕猴桃AdPDC1基因的拟南芥发芽率和根长显著的低于野生型株系，说明猕猴桃AdPDC1与植物激素ABA呈现负相关的关系。因此，AdADH1基因及含有所述的AdPDC1基因的重组表达载体可在创制耐涝新种质和/或植物品种改良中应用。
摘要（英）：

The invention discloses a kiwi fruit AdPDC1 gene and application. Kiwi fruit AdPDC1 is protein provided with an amino acid sequence represented by SEQIDNO.2 in a sequence table, and the encoding gene of the kiwi fruit AdPDC1 is provided with a DNA sequence of SEQIDNO.1 representing the AdPDC1 gene. Waterlogging damage or salt damage induces expression of the kiwi fruit AdPDC1 gene. Compared with wild type groups, the waterlogging resistance capacity of transgenic arabidopsis thaliana having AdPDC1 over-expression is remarkably improved. It is shown that the kiwi fruit AdPDC1 plays an important role on plants in the process of improving waterlogging damage resistance. In addition, under the ABA treatment condition, the transgenic kiwi fruit AdPDC1 gene is remarkably smaller than wild type strains in arabidopsis thaliana germination rate and root length, and it is shown that the kiwi fruit AdPDC1 gene and plant hormones ABA produce the relationship of negative correlation. Therefore, the AdPDC1 gene and a recombinant expression vector containing the AdPDC1 gene can be applied to development of novel germplasm resistant to waterlogging and/or variety improvement of the plants.

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