The invention provides a preparation method of a photosensitive copolymer and carbon nano tube compounded molecularly imprinted sensor and relates to the technical field of combination of polymer material sciences and electrochemical sensors. A molecularly imprinted compound self-assembling body is obtained by compounding and assembling a photosensitive copolymer, a carbon nano tube and a template molecule; then the molecularly imprinted compound self-assembling body is dipped and coated on the surface of a modified electrode; and after ultraviolet radiation and cross linking, the template molecule is eluted to obtain the molecularly imprinted electrochemical sensor. A preparation process of the preparation method is completely carried out in a water phase and has the characteristic of environmental friendliness. The prepared photosensitive copolymer and carbon nano tube compounded molecularly imprinted sensor has excellent sensitivity and a selection and identification capability, and can be repeatedly used for a plurality of times.