The invention discloses a 3- glucamine-5-phenyl-1,2,4-triazole derivative described by formula (I) which has anticancer activities, preparation method and uses thereof. The 3- glucamine-5-phenyl triazole derivative is prepared by adding benzoyl isothiocyanate, and acyl-glucosamine in solvent -1, stirring the solution to make them fully reacting with each other under a reflux condition, decompressing for evaporating the solvent-1 to obtaining solid intermediate, then adding solvent-2, and hydrazine hydrate, reacting under temperature of 35-80 DEG C until the reaction ends, decompressing for evaporating solvent-2, and purifying the resultant. The 3- glucamine-5-phenyl-1,2,4-triazole derivative has an good inhibition effect on the liver cancer HepG2 cell and can be used for preparing anti-tumor drugs.