The invention relates to a probe 96 for selecting a bacterial colony in a high-flux mode. The probe 96 is composed of an upper body and a lower body. Ninety-six vertical piston rod passageways in an array with 8 lines in the longitudinal direction and 12 lines in the transverse direction are distributed in the upper body. A piston rod is arranged in each piston rod passageway, and therefore an upper sealed cavity and a lower sealed cavity are formed in each piton rod passageway. The 8 longitudinal upper sealed cavities in each longitudinal line are communicated with one another, connected with first electromagnetic valves through transverse air inlets and used for controlling the opening and closing of the transverse air inlets. The 12 transverse lower sealed cavities in each transverse line are communicated with one another, connected with second electromagnetic valves through longitudinal air inlets and used for controlling the opening and closing of the longitudinal air inlets. Ninety-six selecting needle passageways arrayed corresponding to the piston rod passageways in the upper body are distributed in the lower body. A selecting needle is arranged in each selecting needle passageway. A reset spring is arranged between each selecting needle passageway and the corresponding selecting needle. The first electromagnetic valves and the second electromagnetic valves are connected with an air pump. The probe 96 is used in cooperation with an operation tail end of an automatic machine or robot for selecting the bacterial colony, and the bacterial colony is selected automatically.