The invention provides a fur cortisol hormone extraction method. The method comprises the following steps: step one, placing a fur sample into isopropyl alcohol for performing vibration washing, and then drying at the temperature of 68 to 72 DEG C for 3.5 to 4.5 hours, so as to obtain the washed fur; step two, grinding the washed fur by using a ball-milling instrument to obtain the ground fur; step three, enabling the ground fur to contact with methyl alcohol at the temperature of 75 to 85 DEG C for 15 to 17h, and then centrifuging to obtain liquid supernatant. By using the method provided by the invention, the extraction volume of cortisol hormone is increased, the sample processing time is shortened, and the extracting efficiency of cortisol is improved.