The invention provides a detection kit for norovirus antibodies EILISA (enzyme-linked immuno sorbent assay), and belongs to the technical field of biological medicines. The detection kit comprises an elisa plate for recombining norovirus VLPs (virus likeparticles) serving as antigen coating. The detection kit of the prepared norovirus is high in specificity, high in sensitivity, short in consumed time, low in using cost and easy and convenient to operate; requirements on purity of samples to be detected are low; and the detection kit is particularly suitable for clinical detection of norovirus infection.