The invention belongs to the field of food microbe safety detection, and provides a primer group for multi-PCR detection of Staphylococcus aureus and salmonellae in a food, a kit and a detection method. The multi-PCR detection primer group, the kit and the detection method can realize the technical effects of wide detection coverage range, strong specificity and high sensitivity through combined utilization of species specificity discriminating genes and drug resistance related genes of Staphylococcus aureus and salmonellae, and have the advantages of short detection time, simple operation, low device requirements, high flux, saving of a large amount of manpower, material resources and financial resources, suitableness for rapid detection requirements, and easy popularization and application in practical production.