利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN105177136A 一种筛查荷斯坦奶牛白细胞粘附缺陷病携带者的分子检测方法 无效 - 驳回

一种筛查荷斯坦奶牛白细胞粘附缺陷病携带者的分子检测方法
摘要：
本发明公开了一种筛查荷斯坦奶牛白细胞粘附缺陷病携带者的分子检测方法。本发明提供了一种鉴定荷斯坦牛是否携带BLAD的竞争性等位基因特异性PCR成套引物，为如下1)或2)：1)由引物1、引物2和引物3组成；2)由引物4、引物5和引物3组成；所述引物1的核苷酸序列为序列1；所述引物2的核苷酸序列为序列2；所述引物3的核苷酸序列为序列3；所述引物4的核苷酸序列为序列4；所述引物5的核苷酸序列为序列5。本发明利用KASP技术，通过设计一组针对BLAD突变位点的引物，进行反应，经过约1.5小时后通过配套的软件进行简单识别分析，即可准确获得所有待测样品的基因型，从而实现筛查BLAD携带者的目的。
摘要（英）：

The invention discloses a molecular detection method for screening Holstein BLAD (bovine leukoeyte adhesion defieieney) disease carriers. The invention provides a competitive allele-specific PCR (polymerase chain reaction) set primer used for identifying whether the Holstein carries BLAD or not. According to the method, (1) the primer consists of a primer 1, a primer 2 and a primer 3, or (2) the primer consists of a primer 4, a primer 5 and a primer 3; the nucleotide sequence of the primer 1 is sequence 1; the nucleotide sequence of the primer 2 is sequence 2; the nucleotide sequence of the primer 3 is sequence 3; the nucleotide sequence of the primer 4 is sequence 4; the nucleotide sequence of the primer 5 is sequence 5. A KASP (kompetitive allele-specific PCR) technology is used; a reaction is performed by designing a group of primers aiming at the BLAD mutation sites; after about 1.5 hours, a simple discriminatory analysis is performed through matched software; the genotypes of all samples to be detected can be accurately obtained, so that the goal of screening the BLAD carriers is achieved.

0/0