The invention relates to the field of disassembly and assembly of maglev trains and provides a disassembly and assembly device for levitation chassis of the middle-low speed maglev trains. The disassembly and assembly device is located above a foundation with running rails, and the length direction of the running rails is perpendicular to the length direction of maglev rails. The disassembly and assembly device comprises a base and a supporting table located above the base. A lifting mechanism is arranged between the supporting table and the base. A running wheel assembly is arranged on the base. Lifting rails located between the two adjacent maglev rails are arranged on the supporting table, and the supporting table and the two adjacent maglev rails are supported and fixed through two or more locking assemblies. The disassembly and assembly device serves as a part of the maglev rails normally and guarantees smooth passing of the trains; when the disassembly and assembly device needs to be used for disassembly and assembly, the lifting rails can be driven by the lifting mechanism to move in the vertical direction, the disassembly and assembly device can conveniently move on the ground through the running wheel assembly so as to realize disassembly and assembly of the levitation chassis of the trains without excessive assist of other tools, and use is convenient.