The invention discloses Trichoderma longibranchiatum, collected in China General Microbiological Culture Collection Center on 14th January, 2014 under CGMCC No. 8723. Fungal cell wall degrading enzymes such as chitinase and glucanase generated by the Trichoderma longibranchiatum may dissolve pathogenic cell walls and permeate in the cells to form a great amount of branched and sexual structures, so that pathogenic fungi are dead due to the suppression of their growth and anthracnose in strawberry is controlled. The Trichoderma longibranchiatum is efficient in suppressing the pathogenic fungi of the anthracnose in strawberry, reducing occurrence of the anthracnose in strawberry and replacing chemical pesticides to suppress the anthracnose in strawberry.