The invention belongs to the field of detection of traditional Chinese medicine preparations and particularly relates to a detection method of a medicine preparation having the effects of nourishing blood, relieving restlessness, tranquilizing mind and calming nerves. According to the detection method, spinosin in mind and nerve tranquilizing tablets can be extracted to a great degree by selecting an extraction solvent, extraction time, an extraction method and the like; the separation effect of the spinosin in the mind and nerve tranquilizing tablets and other chemical components can be best by selecting a chromatographic column, a gradient elution method and the like, and accordingly the spinosin content in the mind and nerve tranquilizing tablets is accurately determined. The detection method is accurate, sensitive, simple and convenient and good in stability and reproducibility, an HPLC chromatogram is good in peak pattern, accordingly the spinosin content in the mind and nerve tranquilizing tablets is accurately determined, and the detection method is beneficial to effective control on the quality of the medicine and improvement of the safety and stability of the medicine in use.