利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN105331579A 人睾丸间质干细胞的分离、培养方法及其用途 无效 - 撤回

人睾丸间质干细胞的分离、培养方法及其用途
摘要：
本发明公开了一种人睾丸间质干细胞的分离、培养方法及其用途。本发明将组织活检分离的梗阻性无精症病人睾丸标本用IV型胶原酶消化，过滤消化后的细胞悬液以去除组织团块，获得人睾丸细胞，用CD51抗体标记人睾丸细胞，利用流式细胞仪进行分选，收集表达CD51的细胞，从而分离出CD51阳性的人睾丸间质干细胞。本发明还提供了通过上述方法分离获得的CD51阳性的人睾丸间质干细胞，该干细胞的培养方法及其在制备治疗睾酮水平低下导致的相关疾病的药物中的应用。
摘要（英）：

The invention discloses a separation and culture method and application of human testis mesenchymal stem cells. The separation method comprises the steps that an obstructive azoospermia patient testis specimen which is processed through tissue biopsy separation is digested with type IV collagenase, digested cell suspension is filtered to remove tissue clumps, and human testis cells are obtained; the human testis cells are marked with a CD51 antibody, separation is performed with a flow cytometer, the cells expressing the CD51 are collected, and then the human testis mesenchymal stem cells with the positive CD51 are separated. The invention further provides the human testis mesenchymal stem cells which are obtained through the separation method and have the positive CD51, the culture method of the stem cells and application of the stem cells in preparation of medicine for treating related diseases caused by low testosterone level.

0/0