The invention is used for the field of food microorganism safe detection, and provides a primer group used for multiple PCR detection by aiming at salmonella and Shigella in food, a kit and a detection method thereof. The primer group used for the multiple PCR detection, the kit and the detection method can combine and use species-specificity discrimination gene and drug resistance-related gene, and the technical effects of wide detection cover scope, strong specificity and high sensitivity can be realized, detection time consumption is short, operation is simple, equipment requirement is low, flux is high; manpower, material resource and financial resource can be saved, the primer group used for the multiple PCR detection, the kit and the detection method are suitable for rapid detection requirement, and are easily popularized and applied in practical production.