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发明公开
CN105349557A 一种苹果酸酶基因RKME2及其重组表达载体 有权

一种苹果酸酶基因RKME2及其重组表达载体
摘要：
本发明公开了一种从红冬孢酵母（Rhodosporidium kratochvilovae）YM25235中分离的编码苹果酸酶的核苷酸序列，其核苷酸序列如SEQ ID NO:1所示，该基因编码的氨基酸序列如SEQ ID NO:2所示，通过构建重组载体并在大肠杆菌BL21中高效表达，表达产物具有苹果酸酶功能。
摘要（英）：

The invention discloses a nucleotide sequence which is separated from Rhodosporidium kratochvilovae YM25235 and is used for coding a malic enzyme. The nucleotide sequence is shown in the formula SEQ ID NO: 1. An amino acid sequence coded by the nucleotide sequence is shown in the formula of SEQ ID NO: 2. Through construction of a recombinant vector and efficient expression of the recombinant vector in escherichia coli, the expressed product has malic enzyme functions.

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