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发明授权
CN105349562B 表达猪细小病毒VP2蛋白的重组载体、重组菌及其应用 有权

表达猪细小病毒VP2蛋白的重组载体、重组菌及其应用
摘要：
本发明提供表达猪细小病毒VP2蛋白的重组载体、重组菌及其应用，属于生物技术领域。表达猪细小病毒VP2蛋白的重组载体，是将猪细小病毒VP2蛋白编码基因插入原核表达载体pEASY‑blunt E1后得到。本发明还提供表达猪细小病毒VP2蛋白的重组菌，是将所述重组载体导入大肠杆菌后获得。本发明表达猪细小病毒VP2蛋白的重组载体，导入大肠杆菌后，能够可溶性表达VP2蛋白，该VP2蛋白具有血凝性和优异的抗原性，有望应用于制备猪细小病毒疫苗。采用本发明重组菌能够高效制备猪细小病毒VP2蛋白，生产成本低、操作简单、不操作猪细小病毒具有更好的生物安全性。
摘要（英）：

The invention provides a recombinant vector and a recombinant strain for expressing PPV (porcine parvovirus) VP2 protein and applications of the recombinant vector and the recombinant strain and belongs to the technical field of biology. The recombinant vector for expressing the PPV VP2 protein is prepared by inserting a PPV VP2 protein coding gene into a prokaryotic expression vector pEASY-blunt E1. The invention further provides the recombinant strain for expressing the PPV VP2 protein. The recombinant strain for expressing the PPV VP2 protein is prepared by introducing the recombinant vector into escherichia coli. The recombinant vector for expressing the PPV VP2 protein can realize soluble expression of the VP2 protein after introduction into the escherichia coli, and the VP2 protein has the hemagglutinating character and excellent antigenicity and is expected to be applied to preparation of a PPV vaccine. With the adoption of the recombinant strain, the PPV VP2 protein can be effectively prepared, the production cost is low, the operation is simple, the PPV is not operated, and better biological safety is realized.

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