The invention discloses an AC and Dc mixed micro-grid operation optimization method based on time-domain rolling control. The method comprises: (1) establishing an AC and DC mixed micro-grid linear model, (2) establishing a distributed power supply output prediction and AC and DC load prediction model, (3) establishing an optimization target function comprising robustness and economy performance, and selecting appropriate optimization targets according to different operation sates of grid-connected operation and off-grid operation of an AC and DC mixed micro-grid, (4) through adoption of a time-domain rolling control method, performing optimization control on a target power distribution network, and (5) performing the rolling time-domain control, and repeating the steps (2)-(4) at t+1 moment until the control ends. Influences brought by renewable energy output power, an energy price fluctuation and a load prediction error are eliminated trough feedback corrections, and, aiming at the different operation states of the AC and DC mixed micro-grid, the different optimization targets are adopted to improve the robustness in the off-grid operation state and the economy performance in the grid-connected operation state.