The present invention provides a reflective film with a moth eye structure and a preparation technology thereof. The reflective film provided by the invention comprises a PET layer, a reflective structure layer and a moth eye anti-reflection structure layer. Through the geometric optics principle, the reflective structure layer is configured to take a pyramid structure with three vertical surfaces as a reflective structure. Through the bionics principle, the moth eye anti-reflection structure layer is configured to take a moth eye micro-nano structure as an anti-reflection layer. Compared with the traditional reflective film, the reflective film provided by the invention has a better reflective effect. Through adoption of reel-to-reel UV two-sided rolling technology (R2R UV-NIL), the preparation technology provided by the invention comprises adhesive dripping, filling, UV illumination and demolding. According to the invention, different structure layers may be printed on the surfaces at two sides of the PET layer; and moreover, the preparation technology is simple, and the fast and large-area commercialization requirement with high resolution and high throughput may be satisfied.