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发明公开
CN105400721A 一种快速筛选具有抗菌活性芽孢杆菌的方法 无效 - 驳回

一种快速筛选具有抗菌活性芽孢杆菌的方法
摘要：
本发明涉及微生物筛选领域，具体公开了一种快速筛选具有抗菌活性芽孢杆菌的方法。利用高温处理快速除去杂菌，并利用指示菌进行琼脂覆盖，通过观察抑菌圈筛选具有抗菌活性芽孢杆菌。可在35～42℃的培养条件下，在琼脂覆盖后培养6～12h内观察到抑菌圈，实现具有抗菌活性芽孢杆菌的高效筛选，整个筛选过程在48h内即可完成，大大简化了筛选的程序，降低了劳动强度。且本方法所述方法只利用了几种较为简单的培养基，无需滤纸片、牛津杯等试验材料，筛选成本低。
摘要（英）：

The invention relates to the field of microbe screening and specifically discloses a method for rapid screening of Bacillus spp with antibacterial activity. According to the invention, high temperature treatment is carried out to rapidly eliminate competitors, agar overlaying is realized via an indication bacterium, and Bacillus spp with antibacterial activity is screened by observing an inhibition zone; and under a culture condition of 35 to 42 DEG C, the inhibition zone can be observed within 6 to 12 h of culture after agar overlaying, so high-efficiency screening of Bacillus spp with antibacterial activity is realized, the whole screening process can be completed within 48 h, screening procedures are substantially simplified, and labor intensity is mitigated. The method only utilizes a plurality of simple medium and does not need test materials like filter paper and an Oxford cup, so screening cost is low.

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