The invention discloses a method for enhancing the cold resistance of Kandelia obovata. According to the method, an artificial accurate phased cooling method is used for enhancing the cold resistance of the Kandelia obovata, so that the survival rate of the Kandelia obovata in a low-temperature environment can be increased, and a transplanting process of the Kandelia obovata to the high latitude is promoted. The core concept of the technical scheme is as follows: the environment temperature is reduced continuously in a seedling culture process while the seedling culture effect of the Kandelia obovata is guaranteed, the adaptability of the Kandelia obovata to the low temperature is improved gradually, and the cold resistance of Kandelia obovata is improved. According to the method, Kandelia obovata seedlings are promoted to perform vigorous photosynthesis by means of a high temperature close to the outside before the environment temperature is adjusted to be low, and nutrients, namely, osmotic adjustment substances including sugar and amino acid facilitating relief of low-temperature stress are generated; then through phased gradient cooling, osmotic substance accumulation can be promoted, the ratio of bound water to free water is increased, the content of unsaturated fatty acid in membrane lipid is increased, phase change of a biofilm is prevented effectively, and the cold resistance of the Kandelia obovata is enhanced.