The invention discloses anti-inflammation application and an extraction method of sesibricin in libanotis buchtormensis, and belongs to the technical field of natural product chemistry. Sesibricin has the anti-inflammation effect and can be applied to preparation of an anti-inflammation drug. The extraction method of sesibricin comprises the steps that coarse libanotis buchtormensis powder is taken and extracted with 95% ethyl alcohol through cold soaking to obtain an ethanol extract; 2, Soxhlet extraction is performed on the obtained extract through petroleum ether (at the temperature of 60 DEG C-90 DEG C), and solvent is recycled to obtain a petroleum ether extract; 3, the petroleum ether extract is separated through silica gel column chromatography, an eluant in which the ratio of the petroleum ether (at the temperature of 60 DEGC-90 DEG C) to ethyl acetate is collected, standing is performed to separate out faint yellow precipitates, after the precipitates are refined, white needle crystals are obtained, and the crystals are identified to be sesibricin through structural identification. The extraction method has the advantages of being mild in extraction condition, easy and convenient to operate and low in cost.