A singlet-oxygen microscopy imaging method on a confocal laser scanning microscopy is provided. The method is characterized in that an objective lens of the confocal laser scanning microscopy is provided with two ends, one of the two ends is a sample end facing a sample to be detected, the other end is a laser inlet end, a dichroic mirror is disposed before the laser inlet end, the dichroic mirror separates singlet-oxygen infrared luminescence returned from the sample end of the objective lens from luminescence with other wavelengths of the sample, the singlet-oxygen infrared luminescence reaches one optical detector of the confocal laser scanning microscopy without passing through a scanning head of the confocal laser scanning microscopy, thus achieving imaging, and by adoption of the method, luminescence intensity imaging and luminescence lifetime imaging of singlet oxygen can be obtained on the confocal laser scanning microscopy, thus facilitating research on interactions among the singlet oxygen, the environment and organisms.